28.12.02

Labdane

Labdane.


Wikipedia, the free encyclopedia




Labdane is a natural bicyclic diterpene. It forms the structural core for a wide variety of natural products collectively known as labdanes or labdane diterpenes. The labdanes were so named because the first members of the class were originally obtained from labdanum, a resin derived from rockrose plants.
A variety of biological activities have been determined for labdane diterpenes including antibacterial, antifungal, antiprotozoal, and anti-inflammatory activities.


by Wikipedia, the free encyclopedia


27.12.02

UNIVERSITY AND LABDANUM OR LADANUM FROM CISTUS CRETICUS

SEARCH FOR LABDANUM FROM CISTUS CRETICUS (ONLY).

22.11.02

UNIVERSITY OF ATHENS NATIONAL& KAPODISTRIAN

http://www.uoa.gr/
http://www.uoa.gr/uoauk/uoaindex.htm

PROFESSOR

Costas Demetzos
School of Pharmacy,

National and Kapodistrian University of AthensPanepistimiopouli
Zographou
Athens 157 71,
Greece
TEL: 210 727-4596 Fax: 210 727-4596
E-mail:
demetzos@pharm.uoa.gr

site : http://www.pharm.uoa.gr/cv/cveng/demetzos.htm

22.10.02

ARISTOTLE UNIVERSITY OF THESSALONIKI




PROFESSOR
Angelos K. Kanellis (B.S., M.Sc., Ph.D.)
Biotechnology of Pharmaceutical Plants

Contact
Angelos K. Kanellis (B.S., M.Sc., Ph.D.)
Group of Biotechnology of Pharmaceutical Plants
Laboratory of Pharmacognosy
Department of Pharmaceutical Sciences

Aristotle University of Thessaloniki
541 24 Thessaloniki,
Greece
Tel (Office):
Lab:
FAX:
Email:

(+30-2310) 997656
(+30-2310) 997619
(+30-2310) 997662
kanellis@pharm.auth.gr





GROUP OF BIOTECHNOLOGY OF PHARMACEUTICAL PLANTS

Lastly, the group is working on secondary metabolism and on biotechnology of medicinal plants. Regarding the first, a cDNA coding for phenylalanine ammonia-lyase (PAL) has been cloned and characterized and studied from melon fruit. Other genes include dehydroflavone reductase from grape and a number ESTs from citrus flavedo tissues.

The lab is working on medicinal plant Cistus creticus spp. creticus which produces a number of diterpenes exhibiting anticancer action. Current activities include:
a) Cloning and gene expression studies of enzymes participating in the biosynthesis of diterpenes in Cistus spp. 2) EST analysis of glandular trichomes, 3) isolation of transcription factors controlling glandular trichome initiation and morphogenesis (abstract 56) and 4) isolation and characterization of Cistus creticus glandular trichome specific genes (abstract 57). The group has developed and published a method of isolation of total RNA and DNA from Cistus creticus (Anal. Biochem. (2004) 328:90-92) and isolated and functionally characterized two full-length cDNA clones for geranylgeranyl diphosphate synthase (GGPPS) and a full-length cDNA encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), 4 cDNA clones coding for sesquiterpene synthases (from which one has been fully functional characterized), a full length cDNA for copalyl diphosphate synthase (which has been functional characterized) and a partial cDNA for kaurene synthase (KS) from Cistus creticus spp. creticus. In addition, antibodies have been raised against GGPPS and KS. 2022 ESTs have been sequenced from a cDNA trichome specific libarary and DNA microarrays have been successfully applied to isolate trichome specific genes.

MORE

22.9.02

UNIVERSITY OF CRETE


Haralambos E. Katerinopoulos
Professor of Organic Chemistry

Tel: +30 2810 545026
Fax: +30 2810 545001



E-mail:
kater@chemistry.uoc.gr

Link to Group page:
http://www.chemistry.uoc.gr/katerinopoulosEducation

1 POST-GRADUATE
Analysis of natural products from plants from the floral of Crete Rosmar
ιnus officinalis L, Origanum dictamnus L και Cistus creticus ssp. creticus (L) Greuter et Burdet and elucidation of the structure of a new component of the essential oil of C. creticusCreticus

Georgia Pagona
005pagona July 2005


The subject of this thesis is the study of the components of the essential oils from three species namely, Rosmarinus officinalis L., Origanum dictamnus L. and Cistus creticus ssp. creticus (L.) Greuter et Burdet which are common plants of the flora of Crete. The essential oils contain substances, which originate via the secondary metabolism of plants, and are used for the interaction of the plants with their environment. These components are vital for some basic functions of the plants such as reproduction and protection from insects and parasites. Exploitation of these properties could lead to safer and more environmentally friendly methods for crop protection. The first part of this thesis includes the analysis of the components of the essential oils from the aforementioned plants, the determination of their chemical structure and the classification of each essential oil according to the area of origin. The essential oil, as well as a number of extracts of Rosmarinus officinalis L. in solvents of increasing polarity, were isolated and their components identified and tested by N.AG.R.E.F. (Plant Protection Institute-Heraklion), as biological agents in pest control. In the second part, the elucidation of the structure of a new compound, isolated from the essential oil of the resin from Cistus creticus ssp. creticus (L.) Greuter et Burdet, is presented. This component was initially identified as a labdane analog, namely, 1,1,4a,6-tetramethyl-5-methylene-1,2,3,4,4
α,5,8,8α-octahydronaphthalene. Based on the data from one- and two-dimensional NMR spectroscopy the structure of the compound was redefined as that of an eremophilene isomer with the IUPAC name 1,2,3,4,6,7,8,8aoctahydro- 1,8a-dimethyl-7-(1-methylethenyl)naphthalene.How to view this document Display the whole document in one of the following formats.Portable Document Format 1681716 bytes.You are granted permission for the non-commercial reproduction, distribution, display, and performance of this document in any format, BUT this permission is only for a period of 45 (forty-five) days from the most recent time that you verified that this document is still available from the University of Crete under terms that include this permission. All other rights are reserved by the author(s).

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Send email to dlib@libh.uoc.gr

FULL TEXT
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2 POST-GRADUATE

S
ynthetic Approaches towards the Drimane Sesquiterpenes cis- and trans-1,1,4
α,6-tetramethyl-5-methylene-1,2,3,4,4α,5,8,8α-octahydronaphthalenes .
Konstantinos Hatzellis

2002hatzellis July 2002


Labdanum (Cistus creticus, ssp creticus L., Greuter et Burdet) is a perennial shrub that grows in the Mediterranean area, especially in Crete. During the hot summer days its leaves secrete a thick, aromatic resin, called by the locals “ladano”. One of the components isolated from the essential oil of the resin was assigned the structure of a drimane type sesquiterpene namely 1,1,4a,6-tetramethyl-5-methylene-1,2,3,4,4a,5,8,8a-octahydro-napthalene (figure 1). Given that the stereochemistry of the fusion of the decalin ring had not been specified, the aims of the present project were twofold: a) The design of a flexible synthetic pathway towards the target molecules, which upon simple synthetic deviations would lead to both cis / trans isomers. b) The total synthesis of both targets and the comparison of their spectral profiles with those of the natural product. During this project the first target was attained and the second one was realized to the point of total synthesis of the cis isomer in 85% purity. Comparison of spectroscopic data of the synthesized cis-isomer with those from the literature on the trans analog as well as the natural product, indicates that the latter has a different structure than either the cis or trans isomers.How to view this document Display the whole document in one of the following formats. Portable Document Format 2360141 bytes.(compressed on disk, will be sent uncompressed) You are granted permission for the non-commercial reproduction, distribution, display, and performance of this document in any format, BUT this permission is only for a period of 45 (forty-five) days from the most recent time that you verified that this document is still available from the University of Crete under terms that include this permission. All other rights are reserved by the author(s).

This server operates at the University of Crete at Heraklion.

Send email to dlib@libh.uoc.gr

FULL TEXT
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22.8.02

INSTITUTE OF OLIVE TREE AND SUBTROPICAL OF CHANIA

SITE
http://www.nagref-cha.gr/indexen.html

Costas Economakis
Email: ceconom@nagref-cha.gr
Tel: 28210 83447

Costas Tzerakis
Agronomist,

Email: ctzerakis@nagref-cha.gr
Tel: 28210 83448

Sofia Saloustrou
Laboratory Technician
Tel: 28210 83429

10.9.99

Plants of the Bible and the Quran

FIGS, DATES LAUREL AND MYRRH 
Plants of the Bible and the Quran 
LYTTON JOHN MUSSELMAN 
Foreword by GARRISON KEILLOR 

Ladanum 

ONE OF THE MOST BEAUTIFUL shrubs in the pine forests of the Middle East are species of Cisius, the source of the resin known as ladanum (or labdanum). This resin has an ancient heritage, being cited in the first book of the Bible, and it has a long history of use: "Then their father Israel [Jacob] said to them [Joseph's brothers), 'If it [the trip to Egypt) must be, then do this: Put some of the best products of the land in your bags and take them down to the man as a gift— a little balm and a little honev, some spices and myrrh [lote], some pistachio nuts and almonds' " (Genesis 43:11, ΝIV); and, "As they sat down to eat their meal, they looked up and saw a caravan of Ishmaelites coming from Gilead. Their camels were loaded with spices, balm and mvrrh [lote] and they were on their were to take them down to Egypt" (Genesis 37:25, NIV). The Hebrew lote should not be confused with the Arabic lore (see "Thornbush"). 

The cargo of the Ishmaelites is of special interest to the edmobotanist. It is unfortunate that the Hebrew word lote is translated as "mvrrh" in these two verses, while in the remaining 11 occurrences in both KJ V and ΝIV, mvrrh is more accurately used for the Hebrew mor. 

What is lote? One possibility is the resin of species of Cisrus, a shrub common in the region east of the Jordan River known as Gilead. The meaning of the word in Hebrew implies something sticky, characteristic of this product. 
Pink cistus,showing the abundant glandular hairs that cover the plant. These produce a sticky, fragrant resin that is the basis of ladanun

Although a small area, Gilead is geologically and ccologically diverse, stretching from the margins of the Jordan Valley and the peaks along the Great Rift Valley to the edge of the badia (Arabic for steppe). In ancient times, parts of Gilead were covered with forests. These forests were the southernmost extension of the Mediterranean woodlands and the southern edge of the range of the Aleppo pine (Pinus halepensis). Two species of Cistus are common in the pine forest, C. crericus and C. salvifolius. They are easily distinguished by their flower color. The large pink flowers of C. creticus and the slightly smaller but equally beautiful white flowers of C. salvifolius appear in May. On a hot day, the fragrant resin of the plants is noticeable. On close examination, you can see the numerous hairs that cover the leaves and young stems of both species. The resinous ladanum will stick to your hands if you collect leaves (Langenheim 2003). The fragrant resin of Cisrus has been used for millennia to produce incense. The resin of related species is still collected in parts of Greece. It can be harvested in a variety of ways. One ancient method is to comb the hair of goats that graze in plant communities where Cistus is abundant. Another is by dragging a rake with long, leather tines across the shrubs at the hottest time of day, and when the resin is dry, removing it from the rake tines (Baumann 1993).

Like many other plants used for incense, ladanum has medicinal value. The resin is used as a balm to reduce inflammation of the skin. 

Recent research on the biochemistry of the plant has shown the efficacy of compounds in the plant for dermatological disorders (Danne et al. 1993), which may be due in part to the antimicrobial activity of compounds in the leaf (Demetzos et al. 1997, 1999). Research in Turkey shows that of the seven plants used as folk remedies for ulcers, the one with the greatest efficacy was C. salvifolius (Yesilada et al. 1999). Jacob's choice of ladanum, therefore, indicates the value placed by people of that time on this plant and its products and possibly reflects on the healing value of the resin. To my knowledge, ladanum docs not have any widespread use among modern Arabs. 
Dibbeen National Forest, Jordan, in May. The dominant tree in the forest is the Aleppo pine, with conspicuous white cistus in the understory.


I have not found any local familiarity with the plants. When I encountered some Bedouin near Anjara, Jordan, feeding their sheep on Cistus, I asked how they used the plant. They simply replied that it was good forage for sheep and goats, and therefore the shrub is absent in heavily grazed areas. 

White cistus flowers in May, Dibbeen National Forest, Jordan. While both the white and pink ι Cistus of the .Middle East can produce resin, the highest quality comes from species in Greece.

Still used to compound incense in the Greek Orthodox Church, ladanum is a little-known product of an attractive flowering shrub of pine woodlands of the Middle East. While it is being examined for its diverse medicinal applications, local people generally are unaware of this or its use as incense. Ladanum bears no relation to laudanum, a tincture of opium that was a popular medicine in Victorian times.